specific amplification of aspergillus fumlgatus dna by polymerase chain reaction

نویسندگان

tahereh shokohi from the department of parasitology, mycology and entomology. mazandaran university of medical sciences, sari, islamic republic of iran

julie c. silver the division of life sciences, department of microbiology and molecular genetics, scarborough campus. university of toronto, ontario. canada

چکیده

invasive aspergillosis (1 is a life-threatening condition in immunocompromised patients. an early diagnosis is of great importance because early treatment may resolve this potentially fatal infection. recently, the polymerase chain reaction (pcr) has been used successfully in detecting specific dna of several pathogen. in this study, nested pcr was used to detect dna specific for a!.pergiflus species isolated from bronchoalveolar lavage (bal) fluid from patients with ta. in single pcr using the outer primers a specific 384-bp fragment was amplified. similarly, y nested pcr with inner primers, a 357-bp fragment was amplified with dna from aspergillus fumigatus but not from the other microorganisms. the southern blot hybridizations confirm the specificity of the pcr procedure for a. fumigatus using the cloned 374-bp pcr product probe. in conclusion, the nested pcr method appears to be quite rapid and specific.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

SPECIFIC AMPLIFICATION OF ASPERGILLUS FUMlGATUS DNA BY POLYMERASE CHAIN REACTION

Invasive aspergillosis (1 is a life-threatening condition in immunocompromised patients. An early diagnosis is of great importance because early treatment may resolve this potentially fatal infection. Recently, the polymerase chain reaction (PCR) has been used successfully in detecting specific DNA of several pathogen. In this study, nested PCR was used to detect DNA specific for A!.pergiflus s...

متن کامل

Haptoglobin genotyping by allele-specific polymerase chain reaction amplification.

We performed haptoglobin (Hp) genotyping by polymerase chain reaction using allele-specific primer-pairs. The major six genotypes of Hp were identified using this method. Among Japanese individuals living in Ehime and Okayama Prefectures, the allele frequencies were estimated to be Hp2 = 0.723 and Hp1s = 0.277. Genotyping of Hp was possible with 0.3 ng of DNA and with 0.125 microliter of blood....

متن کامل

Contaminating DNA in RNA amplification by polymerase chain reaction.

Some suppliers offer kits to isOlate mRNA for amplification without mention of the need for previous DNASe treatment of the samples. Many authom of research papers also omit this treatment [and (or) negative controls] when using such techniques We tested various kits and procedures to determine the DNA contamination in the mRNA preparations. For this we propared human lymphocytes by using Lymph...

متن کامل

Serotype-specific amplification of Rickettsia tsutsugamushi DNA by nested polymerase chain reaction.

Polymerase chain reaction (PCR) with nested primer pairs was used to diagnose scrub typhus and identify the Rickettsia tsutsugamushi serotype. The primer pairs used for PCR were designed on the basis of the nucleotide sequence of the gene that encodes the 56-kDa antigen. Serotype-specific primers were used in the second PCR amplification. Five serovariants, the Gilliam, Karp, Kato, Kawasaki, an...

متن کامل

Specific amplification of Rickettsia tsutsugamushi DNA from clinical specimens by polymerase chain reaction.

Polymerase chain reaction (PCR) was used to detect Rickettsia tsutsugamushi-specific DNA in clinical specimens. The primer pair used for PCR was designed from the nucleotide sequence of the gene encoding the 56-kDa antigen of the Gilliam strain. Theses primers led to a 78-bp fragment by amplifying the genomic DNAs from five serovariants, i.e., the Gilliam, Karp, Kato, Kawasaki, and Kuroki strai...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
medical journal of islamic republic of iran

جلد ۱۳، شماره ۱، صفحات ۶۱-۶۶

کلمات کلیدی

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023